6R9O

Structure of Saccharomyces cerevisiae apo Pan2 pseudoubiquitin hydrolase-RNA exonuclease (UCH-Exo) module in complex with AAGGA RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9159
Spacegroup name	I 2 2 2
Unit cell lengths	90.761, 117.593, 256.568
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	85.565 - 3.319
R-factor	0.2574
Rwork	0.255
R-free	0.30920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4czw
Data reduction software	XDS
Data scaling software	xia2 (1.12)
Phasing software	PHASER (2.8)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.565	3.410
High resolution limit [Å]	3.319	3.319
Rmerge	0.085	1.980
Number of reflections	20744	1496
<I/σ(I)>	12	1
Completeness [%]	100.0	99.8
Redundancy	6.5	6.3
CC(1/2)	0.998	0.624

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.4 M ammonium phosphate