6R9M

Structure of Saccharomyces cerevisiae apo Pan2 pseudoubiquitin hydrolase-RNA exonuclease (UCH-Exo) module in complex with AAGGAA RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9159
Spacegroup name	I 2 2 2
Unit cell lengths	91.414, 118.105, 257.281
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.021 - 3.329
R-factor	0.2399
Rwork	0.237
R-free	0.29530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4czw
Data reduction software	XDS
Data scaling software	xia2 (1.12)
Phasing software	PHASER (2.8)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.170	3.420
High resolution limit [Å]	3.329	3.329
Rmerge	0.097	1.527
Number of reflections	20875	1509
<I/σ(I)>	7.3	1.2
Completeness [%]	100.0	99.9
Redundancy	6.5	6
CC(1/2)	0.983	0.649

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.4 M ammonium phosphate