6R9J

Structure of Saccharomyces cerevisiae apo Pan2 pseudoubiquitin hydrolase-RNA exonuclease (UCH-Exo) module in complex with A7 RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	I 2 2 2
Unit cell lengths	91.480, 116.409, 257.833
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.928 - 3.326
R-factor	0.25
Rwork	0.247
R-free	0.29820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4czw
Data reduction software	XDS
Data scaling software	xia2 (1.12)
Phasing software	PHASER (2.8)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.928	3.420
High resolution limit [Å]	3.326	3.326
Rmerge	0.095	1.821
Number of reflections	20507	1481
<I/σ(I)>	8.3	1
Completeness [%]	99.5	98.6
Redundancy	4.6	4.4
CC(1/2)	0.990	0.590

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.4 M ammonium phosphate