6R85
Structure of Arabidopsis thaliana GLR3.3 ligand-binding domain in complex with L-glutamate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-25 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 |
Unit cell lengths | 35.886, 61.280, 64.103 |
Unit cell angles | 75.19, 75.53, 90.02 |
Refinement procedure
Resolution | 59.000 - 2.000 |
R-factor | 0.2093 |
Rwork | 0.207 |
R-free | 0.26180 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.483 |
Data reduction software | XDS |
Data scaling software | Aimless (0.6.2) |
Phasing software | CRANK2 |
Refinement software | REFMAC (5) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 59.000 | 59.110 | 2.050 |
High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
Rmerge | 0.115 | 0.052 | 0.488 |
Rmeas | 0.138 | 0.063 | 0.584 |
Rpim | 0.076 | 0.035 | 0.318 |
Number of reflections | 33332 | 366 | 2402 |
<I/σ(I)> | 4.9 | ||
Completeness [%] | 96.7 | 97.1 | 94.7 |
Redundancy | 3.2 | 3.4 | 3.2 |
CC(1/2) | 0.990 | 0.992 | 0.834 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 100 mM sodium acetate pH 4.6, 240 mM ammonium sulfate, 30%(w/v) PEG monomethyl ether 2,000. |