6R7R
Crystal structure of the glutamate transporter homologue GltTk in complex with D-aspartate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-23 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.99187 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 116.550, 116.550, 314.770 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.057 - 2.800 |
| R-factor | 0.2335 |
| Rwork | 0.232 |
| R-free | 0.26850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e9s |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.166 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.057 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.111 | 3.768 |
| Number of reflections | 61573 | 4459 |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 99.3 | 98.9 |
| Redundancy | 4.89 | 3.58 |
| CC(1/2) | 0.999 | 0.117 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 20% glycerol, 10% PEG 4000, 100 mM Tris/bicine, pH 8.0, 60 mM CaCl2, 60 mM MgCl2, 0.75% n-octyl-b-D-glucopyranoside |
