6R78
Structure of IMP-13 metallo-beta-lactamase in apo form (loop closed)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2018-02-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9660 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.790, 73.120, 64.210 |
| Unit cell angles | 90.00, 112.67, 90.00 |
Refinement procedure
| Resolution | 59.250 - 2.210 |
| R-factor | 0.1805 |
| Rwork | 0.179 |
| R-free | 0.21640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.409 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.250 | 2.294 |
| High resolution limit [Å] | 2.200 | 2.214 |
| Number of reflections | 25528 | 2538 |
| <I/σ(I)> | 13.47 | |
| Completeness [%] | 98.3 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.998 | 0.734 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.1 M SPG buffer pH 8.0, 25% PEG 1500 |
