6R74

N-terminally reversed variant of FimA E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-16
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	86.550, 86.550, 164.210
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.275 - 1.810
R-factor	0.1903
Rwork	0.189
R-free	0.21890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nkt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.275	43.275	1.860
High resolution limit [Å]	1.810	8.090	1.810
Rmerge	0.167	0.061	1.890
Rmeas	0.176	0.065	2.114
Number of reflections	21875	282	1600
<I/σ(I)>	10	33.58	0.86
Completeness [%]	99.8	98.9	99.8
Redundancy	9.609	9.83	5.003
CC(1/2)	0.998	0.997	0.591

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.1	298	45% NH4SO2 and 0.1M Sodium Malonate buffer pH 3.1