6R6O

Recombinantly produced Kusta0087/Kusta0088 Complex, C32G/wt mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-01-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	44.240, 131.450, 45.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.854 - 1.900
R-factor	0.1832
Rwork	0.182
R-free	0.20580
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	6r6m
RMSD bond length	0.007
RMSD bond angle	0.979
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	REFMAC
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.000	2.000
High resolution limit [Å]	1.900	1.900
Rmeas	0.073	0.960
Number of reflections	21517	2996
<I/σ(I)>	19.2
Completeness [%]	99.6	99.5
Redundancy	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	20-25% PEG3000, 0.1 M Na-Citrate