6R6N
Recombinantly produced Kusta0087/Kusta0088 Complex, C32M/C101M mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-01-01 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 44.680, 130.430, 45.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.660 - 2.000 |
R-factor | 0.2016 |
Rwork | 0.200 |
R-free | 0.23590 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 6r6m |
RMSD bond length | 0.008 |
RMSD bond angle | 1.063 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmeas | 0.123 | 0.736 |
Number of reflections | 18722 | 2465 |
<I/σ(I)> | 13.7 | |
Completeness [%] | 99.6 | 99.4 |
Redundancy | 6.2 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 20-25% PEG3000, 0.1 M Na-citrate |