6R5Z
9-bladed beta-propeller formed by three 3-bladed fragments
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.549, 92.353, 61.432 |
| Unit cell angles | 90.00, 94.95, 90.00 |
Refinement procedure
| Resolution | 38.300 - 1.750 |
| R-factor | 0.18653 |
| Rwork | 0.185 |
| R-free | 0.21173 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ymu |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.722 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.300 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.063 | 0.890 |
| Number of reflections | 59988 | 9362 |
| <I/σ(I)> | 13.5 | 1.55 |
| Completeness [%] | 99.2 | 95.9 |
| Redundancy | 4.67 | 4.44 |
| CC(1/2) | 0.999 | 0.650 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2 M Ammonium fluoride, 20 %(w/v) PEG 3350 |
