6R5Y
8-bladed beta-propeller formed by two 4-bladed fragments
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 97.721, 127.167, 72.958 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.700 - 2.150 |
| R-factor | 0.22445 |
| Rwork | 0.223 |
| R-free | 0.25368 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ymu |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.514 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.700 | 2.280 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.110 | 0.760 |
| Number of reflections | 49886 | 7918 |
| <I/σ(I)> | 10.1 | 1.94 |
| Completeness [%] | 99.4 | 99 |
| Redundancy | 3.7 | 3.51 |
| CC(1/2) | 0.995 | 0.726 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.01M Zinc chloride 0.1M Hepes pH 7 20% w/v PEG 6000 |
