6R5V
The crystal structure of Glycoside Hydrolase BglX inactive mutant D286N from P. aeruginosa in complex with xylotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979257 |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.955, 73.668, 81.444 |
| Unit cell angles | 65.55, 73.46, 69.66 |
Refinement procedure
| Resolution | 45.270 - 1.800 |
| R-factor | 0.16451 |
| Rwork | 0.163 |
| R-free | 0.19654 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tf0 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.674 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.270 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rpim | 0.080 | 0.420 |
| Number of reflections | 123955 | 6063 |
| <I/σ(I)> | 8.1 | |
| Completeness [%] | 96.3 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 100 mM Bis Tris, pH 5.5, 17% PEG 10000 and 100 mM ammonium acetate |
