6R5S
Structure of the SBP FpvC from pseudomonas aeruginosa in complex with Fe(II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-08 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.738 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.010, 44.190, 179.760 |
| Unit cell angles | 90.00, 95.06, 90.00 |
Refinement procedure
| Resolution | 37.620 - 2.750 |
| R-factor | 0.192 |
| Rwork | 0.189 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6r3z |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.620 | 2.910 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 14604 | 4343 |
| <I/σ(I)> | 9.35 | |
| Completeness [%] | 96.5 | |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | PEG 6K, 0.1 M Mes |
