6R45
Crystal structure of eukaryotic O-GlcNAcase HAT-like domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-10 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.965 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.250, 43.520, 59.840 |
| Unit cell angles | 87.52, 85.24, 87.58 |
Refinement procedure
| Resolution | 36.360 - 1.784 |
| R-factor | 0.19351 |
| Rwork | 0.193 |
| R-free | 0.23979 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bmh |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.567 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.590 | 1.848 |
| High resolution limit [Å] | 1.784 | 1.784 |
| Rmerge | 0.070 | |
| Number of reflections | 36731 | 3663 |
| <I/σ(I)> | 8 | |
| Completeness [%] | 89.0 | |
| Redundancy | 1.6 | |
| CC(1/2) | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M HEPES 7.5, 60 mM Sodium potassium-tartrate and 27.5 % PEG 8000 |
