6R44
Structure of the SBP FpvC in complex with Ni2+ ion from P.aeruginosa from P21 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.920, 41.380, 104.620 |
| Unit cell angles | 90.00, 95.85, 90.00 |
Refinement procedure
| Resolution | 43.190 - 1.990 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6r3z |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.190 | 2.050 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Number of reflections | 38967 | 8580 |
| <I/σ(I)> | 6.13 | |
| Completeness [%] | 99.1 | |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 16% PEG 6K, 0.1 M MES |
