6R36
T. brucei farnesyl pyrophosphate synthase (FPPS)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.999740 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 60.490, 60.490, 340.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.650 - 1.670 |
| R-factor | 0.221 |
| Rwork | 0.219 |
| R-free | 0.24290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ryp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.930 |
| Data reduction software | XDS (20180126) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.384 | 1.883 |
| High resolution limit [Å] | 1.668 | 1.668 |
| Rmerge | 0.091 | 1.641 |
| Rmeas | 0.094 | 1.700 |
| Rpim | 0.022 | 0.437 |
| Number of reflections | 28487 | 1424 |
| <I/σ(I)> | 21.7 | 1.9 |
| Completeness [%] | 63.8 | 10.7 |
| Redundancy | 18.3 | 14.4 |
| CC(1/2) | 1.000 | 0.736 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.12 M Cesium chloride, 12 %w/v PEG 3350, 12 % v/v DMSO |
