6R2V

Arabidopsis NF-Y/CCAAT-box complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-07-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.999998
Spacegroup name	P 21 21 21
Unit cell lengths	53.446, 85.525, 130.305
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.322 - 2.503
R-factor	0.2024
Rwork	0.200
R-free	0.24690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4awl
RMSD bond length	0.007
RMSD bond angle	0.890
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX ((dev_3304: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.320	2.900
High resolution limit [Å]	2.500	2.500
Rpim	0.079	0.484
Number of reflections	11940	271
<I/σ(I)>	7.6
Completeness [%]	56.1	8.5
Redundancy	7.7	8.2
CC(1/2)	0.990	0.678

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	294	10% PEG 8000, 5% ethylene glycol, 0.1M MES pH 6.5