6R17

Crystal structure of the SYCE2-TEX12 delta-Ctip 2:2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9786
Spacegroup name	P 1 21 1
Unit cell lengths	88.520, 24.190, 88.480
Unit cell angles	90.00, 115.74, 90.00

Refinement procedure

Resolution	79.739 - 2.424
R-factor	0.2915
Rwork	0.287
R-free	0.32960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	18 amino-acid ideal helices
RMSD bond length	0.005
RMSD bond angle	0.788
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_3126: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.740	2.740
High resolution limit [Å]	2.420	2.420
Rmeas	0.074	1.293
Rpim	0.049	0.914
Number of reflections	8501	424
<I/σ(I)>	10.2	1.2
Completeness [%]	63.2
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	35 % MPD