6R0R
Getah virus macro domain in complex with ADPr covalently bond to Cys34
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-31 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98005 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.011, 46.801, 51.004 |
| Unit cell angles | 90.00, 104.16, 90.00 |
Refinement procedure
| Resolution | 37.370 - 1.450 |
| R-factor | 0.17101 |
| Rwork | 0.169 |
| R-free | 0.19667 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6qzu |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.583 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.370 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.043 | 0.749 |
| Rmeas | 0.051 | 0.895 |
| Rpim | 0.027 | 0.484 |
| Number of reflections | 26026 | 1293 |
| <I/σ(I)> | 20.6 | 2.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.6 | 6.5 |
| CC(1/2) | 0.999 | 0.775 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.9 | 293 | Imidazole-Malate pH 5.9, 38% PEG 4K, 3 mM ADPr, 3 mM Aspartic acid |
