6R0N

Histone fold domain of AtNF-YB2/NF-YC3 in I2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-02-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.999996
Spacegroup name	I 1 2 1
Unit cell lengths	37.194, 57.416, 97.827
Unit cell angles	90.00, 99.36, 90.00

Refinement procedure

Resolution	49.350 - 2.100
R-factor	0.1935
Rwork	0.193
R-free	0.20730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5g49
RMSD bond length	0.014
RMSD bond angle	1.345
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.400	2.160
High resolution limit [Å]	2.100	2.100
Rpim	0.018	0.351
Number of reflections	11924	958
<I/σ(I)>	13.9	2.3
Completeness [%]	99.6	99.2
Redundancy	6.7	6.8
CC(1/2)	1.000	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	8	277	100 mM NaCl, 50 mM Tris-HCl pH 8.0 and 2 mM DTT