6R0M
Histone fold domain of AtNF-YB2/NF-YC3 in P212121
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-11-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.010876 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.023, 74.980, 86.654 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.070 - 2.300 |
| R-factor | 0.202 |
| Rwork | 0.200 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5g49 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.960 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.070 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rpim | 0.215 | |
| Number of reflections | 20843 | 2054 |
| <I/σ(I)> | 12.5 | 3.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.7 | 13.5 |
| CC(1/2) | 0.998 | 0.575 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 30% PEG 3350, 0.1 M MES pH 6.5 and 0.3 M lithium citrate |
