6R0I
Glycogen Phosphorylase b in complex with 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION NOVA |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2017-05-10 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.5419 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.309, 128.309, 115.807 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.820 - 2.400 |
| R-factor | 0.14978 |
| Rwork | 0.147 |
| R-free | 0.19649 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.750 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.820 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 36628 | 3704 |
| <I/σ(I)> | 12.2 | 2 |
| Completeness [%] | 90.2 | 87 |
| Redundancy | 3.6 | 2.6 |
| CC(1/2) | 0.988 | 0.772 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.8 | 289 | 10 mM BES buffer |
