6QZU

Getah virus macro domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.91976
Spacegroup name	P 21 21 21
Unit cell lengths	46.552, 71.358, 94.954
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.800 - 2.000
R-factor	0.196
Rwork	0.194
R-free	0.23108
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gpg
RMSD bond length	0.009
RMSD bond angle	1.304
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.800	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.134	0.748
Rmeas	0.173	0.976
Rpim	0.108	0.619
Number of reflections	22033	6918
<I/σ(I)>	7.3	2.1
Completeness [%]	99.8	99.8
Redundancy	4.5	4.3
CC(1/2)	0.989	0.603

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	293	0,2M Imidazole Malate pH6 30% PEG 4K