6QZT

Crystal structure of Csx1 from Sulfolobus islandicus orthorhombic form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	I 21 21 21
Unit cell lengths	107.230, 118.680, 356.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.320 - 2.930
R-factor	0.198
Rwork	0.196
R-free	0.24500
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.230
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	112.740	3.030
High resolution limit [Å]	2.790	2.790
Number of reflections	38474	1892
<I/σ(I)>	13.6	1.7
Completeness [%]	88.2	68.6
Redundancy	14.1	13.1
CC(1/2)	0.990	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.1M tricine pH 8.0 0.35M NaCl 28% PEG 1000 10% Glycerol