6QZN
H30 MnSOD-3 Mutant III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.09 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 81.900, 81.900, 137.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.910 - 1.640 |
| R-factor | 0.20002 |
| Rwork | 0.199 |
| R-free | 0.22759 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dc5 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.548 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.910 | 1.730 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Number of reflections | 57915 | 3988 |
| <I/σ(I)> | 14.6 | |
| Completeness [%] | 99.9 | |
| Redundancy | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | bicine ammonium sulfate |
