6QZL

Structure of the H1 domain of human KCTD12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9762
Spacegroup name	C 2 2 21
Unit cell lengths	88.320, 91.360, 152.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.680 - 1.980
R-factor	0.2067
Rwork	0.204
R-free	0.24850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qb7
RMSD bond length	0.006
RMSD bond angle	0.770
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.680	2.050
High resolution limit [Å]	1.980	1.980
Number of reflections	43000	4238
<I/σ(I)>	19.92
Completeness [%]	99.5
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	21% PEG Smear Broad -- 0.1M citrate/phosphate pH 5.7