6QYB

Streptomyces lividans Ccsp mutant - H111A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	65.276, 62.202, 65.169
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.250 - 1.180
R-factor	0.1827
Rwork	0.182
R-free	0.19400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ei0
RMSD bond length	0.006
RMSD bond angle	1.399
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.280	1.200
High resolution limit [Å]	1.180	1.180
Rmerge	0.056	0.809
Rmeas	0.061	0.951
Rpim	0.023	0.491
Number of reflections	86182	3562
<I/σ(I)>	17.6	1.2
Completeness [%]	98.1	81.6
Redundancy	6.3	3.3
CC(1/2)	0.998	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.5 M Ammonium sulfate, pH 6.0