6QXX

Pink beam serial crystallography: Lysozyme, 5 us exposure, 14793 patterns merged

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID09
Synchrotron site	ESRF
Beamline	ID09
Temperature [K]	298
Detector technology	PIXEL
Collection date	2017-07-15
Detector	PSI JUNGFRAU 1M
Wavelength(s)	0.8058-0.8262
Spacegroup name	P 43 21 2
Unit cell lengths	79.860, 79.860, 38.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.235 - 1.700
R-factor	0.1539
Rwork	0.151
R-free	0.17960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ftr
RMSD bond length	0.014
RMSD bond angle	1.286
Data reduction software	CrystFEL (0.3.6)
Data scaling software	CrystFEL (0.8.0)
Phasing software	PHASER
Refinement software	PHENIX (1.10.1-2155_9999)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.235	1.761
High resolution limit [Å]	1.700	1.700
Number of reflections	14024	1348
<I/σ(I)>	10.72
Completeness [%]	100.0
Redundancy	192.1
CC(1/2)	0.988	0.789

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.5	277	50mM sodium acetate 3.5, 0.75M sodium chloride, 30% ethylene glycol, 11.25% polyethylene glycol 400