6QX5

Crystal structure of T7 bacteriophage portal protein, 12mer, closed valve

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-31
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9340
Spacegroup name	P 21 21 21
Unit cell lengths	165.850, 191.330, 260.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.230 - 3.600
R-factor	0.28643
Rwork	0.284
R-free	0.33441
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qwp
RMSD bond length	0.006
RMSD bond angle	1.452
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.230	3.730
High resolution limit [Å]	3.600	3.600
Rmerge	0.110
Number of reflections	82010	5972
<I/σ(I)>	8.9
Completeness [%]	89.7
Redundancy	3.6
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M CaCl2, 0.1 M sodium acetate pH 4.6 and 30 % (w/v) PEG 400