6QWW

HEWL lysozyme, crystallized from CuCl2 solution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	KURCHATOV SNC BEAMLINE K4.4
Synchrotron site	KURCHATOV SNC
Beamline	K4.4
Temperature [K]	100
Detector technology	CCD
Collection date	2018-05-28
Detector	MAR CCD 165 mm
Wavelength(s)	0.80241
Spacegroup name	P 43 21 2
Unit cell lengths	78.739, 78.739, 36.774
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.840 - 1.350
R-factor	0.16128
Rwork	0.160
R-free	0.18962
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wld
RMSD bond length	0.010
RMSD bond angle	1.463
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.840	1.385
High resolution limit [Å]	1.350	1.350
Rmerge	0.079
Number of reflections	25990
<I/σ(I)>	15.5
Completeness [%]	99.2
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	CuCl2