6QWV
SARM1 SAM1-2 domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 43 |
| Unit cell lengths | 252.246, 252.246, 49.766 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.400 - 2.470 |
| R-factor | 0.1897 |
| Rwork | 0.189 |
| R-free | 0.22969 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.457 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.400 | 2.510 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Number of reflections | 113145 | |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 99.4 | |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 2% Tacsimate pH=7, 5% v/v 2-Propanol, 0.1 M Imidazole pH=7, 8% w/v polyethylene glycol 3350 |
