6QWM

The Transcriptional Regulator PrfA-A218G mutant from Listeria Monocytogenes in complex with a 30-bp operator PrfA-box motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9677
Spacegroup name	P 43 21 2
Unit cell lengths	78.628, 78.628, 265.122
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.060 - 2.900
R-factor	0.2365
Rwork	0.234
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lej
RMSD bond length	0.003
RMSD bond angle	0.533
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.100	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.137	0.976
Rpim	0.038	0.261
Number of reflections	19313	1891
<I/σ(I)>	18.2	4
Completeness [%]	99.8	99.9
Redundancy	25.7	27.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	Droplets of 4 microl protein-DNA solution (ratio 1:1.3) in 20 mM Tris-HCl pH 8.0, 50 mM NaCl, and 1 mM EDTA at 6 mg per ml were mixed with 2 microl reservoir solution consisting of 8% PEG 8000, 100 mM sodium citrate pH 4.6, 100 mM Magnesium acetate and 20% glycerol.