6QW5

Structure and function of the toscana virus cap snatching endonuclease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97934
Spacegroup name	P 21 21 2
Unit cell lengths	101.957, 106.209, 58.623
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.099 - 1.988
R-factor	0.2052
Rwork	0.203
R-free	0.24160
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.747
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.100	2.090
High resolution limit [Å]	1.980	1.980
Rmerge	0.165	0.772
Rmeas	0.185	0.871
Rpim	0.056	0.376
Number of reflections	36170	6019
<I/σ(I)>	0.1	2.2
Completeness [%]	99.0	93.1
Redundancy	10.8	9.8
CC(1/2)	0.995	0.818

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0,1M Hepes pH 7,5 0,75 M LiSO4