6QW4
Engineered streptavidin variant (ACGR) in complex with the Strep-tag II peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-12 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91841 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 57.160, 57.160, 175.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.720 - 2.100 |
R-factor | 0.2018 |
Rwork | 0.200 |
R-free | 0.23970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rst |
RMSD bond length | 0.013 |
RMSD bond angle | 1.621 |
Data reduction software | MOSFLM (7.0.9) |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER (2.5.6) |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 54.337 | 36.696 | 2.210 |
High resolution limit [Å] | 2.100 | 6.640 | 2.100 |
Rmerge | 0.035 | 0.360 | |
Rmeas | 0.123 | 0.037 | 0.379 |
Rpim | 0.039 | 0.012 | 0.117 |
Number of reflections | 8934 | 344 | 1261 |
<I/σ(I)> | 14.2 | 13.8 | 2 |
Completeness [%] | 100.0 | 99.4 | 100 |
Redundancy | 10 | 8.3 | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | lithium sulfate, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid |