6QW0

Structure and function of toscana virus cap snatching endonucleases

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-03
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97857
Spacegroup name	P 21 21 2
Unit cell lengths	100.847, 107.848, 58.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.553 - 1.500
R-factor	0.2098
Rwork	0.208
R-free	0.23560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qw5
RMSD bond length	0.006
RMSD bond angle	0.739
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.553	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.070	2.497
Rpim	0.042
Number of reflections	99878	4745
<I/σ(I)>	11.6	0.6
Completeness [%]	97.9	94.7
Redundancy	5.6	5.4
CC(1/2)		0.318

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	COUNTER-DIFFUSION		293	0.1 M Hepes pH 7.5 0.75 M LiSO4