6QVV

Structure and function of phenuiviridae cap snatching endonucleases

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-22
Detector	DECTRIS EIGER R 1M
Wavelength(s)	0.9677
Spacegroup name	P 21 21 2
Unit cell lengths	101.004, 109.638, 58.159
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.180 - 2.400
R-factor	0.2038
Rwork	0.201
R-free	0.25560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qw5
RMSD bond length	0.008
RMSD bond angle	0.845
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.180	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.159	1.062
Rmeas	0.182	1.211
Rpim	0.086	0.570
Number of reflections	25558	2672
<I/σ(I)>	7.3	1.4
Completeness [%]	98.7	99.1
Redundancy	4.2	4.4
CC(1/2)	0.993	0.593

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	COUNTER-DIFFUSION		293	0.1 M Hepes pH 7.5 0.75 M LiSO4