6QVS
Unliganded structure of the human wild type Beta-galactoside alpha-2,6-sialyltransferase 1 (ST6Gal1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-30 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.967700 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.816, 94.369, 64.472 |
| Unit cell angles | 90.00, 94.31, 90.00 |
Refinement procedure
| Resolution | 38.039 - 1.600 |
| R-factor | 0.1821 |
| Rwork | 0.181 |
| R-free | 0.20050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4js1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.792 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.040 | 1.657 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.046 | 0.613 |
| Rmeas | 0.051 | 0.684 |
| Rpim | 0.023 | 0.300 |
| Number of reflections | 107819 | 10784 |
| <I/σ(I)> | 17.43 | 2.28 |
| Completeness [%] | 98.9 | 99.09 |
| Redundancy | 5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M Bis-Tris pH 5.5, 0.1 M Ammonium Acetate, 8% (w/v) PEG 20.000 |
