6QVH

Streptomyces lividans Ccsp mutant - H113A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	I 2 2 2
Unit cell lengths	62.200, 65.060, 65.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.670 - 1.280
R-factor	0.1623
Rwork	0.161
R-free	0.18700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ei0
RMSD bond length	0.005
RMSD bond angle	1.330
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.670	1.300
High resolution limit [Å]	1.280	1.280
Rmerge	0.055	1.440
Rmeas	0.040	1.554
Rpim	0.022	0.566
Number of reflections	33353	1526
<I/σ(I)>	23.7	1.4
Completeness [%]	96.7	89.2
Redundancy	7.3	7.3
CC(1/2)	0.998	0.586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	between and 10-15 mg/ml of protein mixed with 1.5 M Ammonium sulfate, 0.1M MES pH 6.0