6QV2
Structure of ATPgS-bound outward-facing TM287/288 in complex with nanobody Nb_TM#2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-15 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 88.600, 113.070, 126.890 |
| Unit cell angles | 83.18, 73.00, 67.37 |
Refinement procedure
| Resolution | 32.590 - 4.230 |
| R-factor | 0.307 |
| Rwork | 0.306 |
| R-free | 0.33000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.900 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 32.590 | 32.590 | 4.320 |
| High resolution limit [Å] | 4.220 | 18.850 | 4.220 |
| Rmerge | 0.052 | 0.010 | 0.351 |
| Rmeas | 0.061 | 0.012 | 0.410 |
| Number of reflections | 18010 | 320 | 48 |
| <I/σ(I)> | 16.12 | 117.77 | 3.25 |
| Completeness [%] | 57.4 | 92.8 | 2.1 |
| Redundancy | 3.533 | 3.734 | 3.583 |
| CC(1/2) | 1.000 | 1.000 | 0.838 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M TRIS, 0.1M NaCl, 30% (v/v) PEG 400 |
