6QV0

Structure of ATP-bound outward-facing TM287/288 in complex with sybody Sb_TM35

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-12
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	166.280, 77.290, 207.180
Unit cell angles	90.00, 112.55, 90.00

Refinement procedure

Resolution	49.190 - 3.120
R-factor	0.245
Rwork	0.244
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.940
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.190	49.190	3.190
High resolution limit [Å]	3.110	13.920	3.110
Rmerge	0.187	0.029	0.940
Rmeas	0.202	0.032	1.021
Number of reflections	55123	1042	203
<I/σ(I)>	9.25	46.33	2.28
Completeness [%]	62.6	95.9	3.2
Redundancy	6.726	6.524	6.596
CC(1/2)	0.998	0.999	0.772

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	0.1M Sodium acetate, 0.025M NaCl, 12% (w/v) PEG 6000