6QUJ
GHK tagged GFP variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-18 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.34 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 85.990, 85.990, 270.140 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.077 - 1.680 |
| R-factor | 0.1742 |
| Rwork | 0.172 |
| R-free | 0.22130 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.422 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.480 | 1.710 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.060 | 2.359 |
| Rpim | 0.009 | 0.471 |
| Number of reflections | 68491 | 68359 |
| <I/σ(I)> | 28.5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 36.1 | |
| CC(1/2) | 1.000 | 0.622 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Mes pH 6.5, 1.2M Ammonium Sulphate, 8% Glycerol |
