6QUI
GHK tagged GFP variant at 17Kev
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.72931 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 86.252, 86.252, 269.535 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.960 - 1.940 |
| R-factor | 0.2019 |
| Rwork | 0.201 |
| R-free | 0.22400 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.402 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.960 | 1.980 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rpim | 0.012 | 0.230 |
| Number of reflections | 45055 | 4402 |
| <I/σ(I)> | 32.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 155.6 | |
| CC(1/2) | 1.000 | 0.886 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Mes pH 6.5, 1.2M Ammonium sulphate, 8% glycerol |
