6QUF
Protein crystallization by ionic liquid hydrogel support: reference crystal of glucose isomerase grown on standard silanized glass
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97779 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 92.522, 98.167, 101.851 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.930 - 1.190 |
| R-factor | 0.10775 |
| Rwork | 0.107 |
| R-free | 0.12943 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oad |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.347 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | Sir2014 |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.930 | 1.220 |
| High resolution limit [Å] | 1.190 | 1.190 |
| Rmerge | 0.042 | 0.166 |
| Rmeas | 0.049 | 0.234 |
| Rpim | 0.030 | 0.166 |
| Number of reflections | 112879 | 522 |
| <I/σ(I)> | 14.7 | 2.6 |
| Completeness [%] | 76.7 | 4.9 |
| Redundancy | 3.4 | 1 |
| CC(1/2) | 0.999 | 0.966 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | Protein concentration 26mg/ml in HEPES 10mM pH 7 and MgCl2 1mM. reservoir: (NH4)2SO4 1.5M |
