6QUD
2-deoxy-galactose reaction intermediate of a Truncated beta-galactosidase III from Bifidobacterium bifidum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976252 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.210, 53.920, 112.620 |
| Unit cell angles | 90.00, 106.59, 90.00 |
Refinement procedure
| Resolution | 41.811 - 2.100 |
| R-factor | 0.1668 |
| Rwork | 0.165 |
| R-free | 0.20860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6quc |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.570 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.108 | 0.635 |
| Number of reflections | 46763 | 4583 |
| <I/σ(I)> | 11.5 | 2 |
| Completeness [%] | 98.0 | 97 |
| Redundancy | 4.2 | 4 |
| CC(1/2) | 0.996 | 0.756 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 23 % PEG 1500 5 mM glucose 10 mM Tris:HCL |
