6QU8

Adenovirus Serotype 26 (Ad26) in complex with sialic acid, pH8.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91587
Spacegroup name	P 21 3
Unit cell lengths	85.920, 85.920, 85.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.960 - 1.190
R-factor	0.14322
Rwork	0.142
R-free	0.17201
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fjn
RMSD bond length	0.011
RMSD bond angle	1.661
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.960	1.220
High resolution limit [Å]	1.190	1.190
Rmerge	0.085
Rmeas	0.088
Number of reflections	67799
<I/σ(I)>	20.7
Completeness [%]	100.0
Redundancy	21.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	0.1 M MIB, 25 % w/v PEG 1500, pH8.0