6QTX

Crystal structure of an Arabidopsis WD40 domain in complex with a flowering transcription factor homolog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-08
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.03
Spacegroup name	P 21 21 21
Unit cell lengths	48.465, 55.127, 103.538
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.660 - 1.950
R-factor	0.1414
Rwork	0.139
R-free	0.18970
RMSD bond length	0.010
RMSD bond angle	1.021
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.660	2.020
High resolution limit [Å]	1.950	1.950
Rmeas	0.035
Number of reflections	20513
<I/σ(I)>	24.92
Completeness [%]	98.2
Redundancy	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	5 mg/mL of COP1 supplemented with 3 to 10 fold molar excess in peptide was mixed with two-fold (v/v) more mother liquor (1:2 ratio; protein:buffer) containing 2 M (NH4)2SO4 and 0.1 M HEPES pH 7.4 or 0.1M Tris pH 8.5.