6QTQ

Crystal structure of an Arabidopsis WD40 domain in complex with photoreceptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-08
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.03
Spacegroup name	P 21 21 21
Unit cell lengths	48.678, 54.993, 102.892
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.000 - 1.300
R-factor	0.1386
Rwork	0.137
R-free	0.17390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5igo
RMSD bond length	0.008
RMSD bond angle	1.082
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.000	1.346
High resolution limit [Å]	1.300	1.300
Number of reflections	68540
<I/σ(I)>	8.3
Completeness [%]	99.7
Redundancy	19.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	5 mg/mL of COP1 supplemented with 3 to 10 fold molar excess in peptide was mixed with two-fold (v/v) more mother liquor (1:2 ratio; protein:buffer) containing 2 M (NH4)2SO4 and 0.1 M HEPES pH 7.4 or 0.1M Tris pH 8.5.