6QT8

Crystal structure of Rea1-MIDAS domain from Chaetomium thermophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97264
Spacegroup name	P 41 21 2
Unit cell lengths	85.853, 85.853, 156.302
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.540 - 2.330
R-factor	0.1831
Rwork	0.181
R-free	0.21210
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.995
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXDE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.540	2.410
High resolution limit [Å]	2.330	2.330
Number of reflections	47603
<I/σ(I)>	14.09
Completeness [%]	99.8
Redundancy	4.2
CC(1/2)	0.999	0.414

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	1.48 M (NH4)2SO4, 5 mM MgCl2 and 10 mM NaI