6QSL
mTFP* closed conformation: I197C-Y200H-Y204H mutant for enhanced metal binding
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-22 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.756, 69.838, 75.199 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.600 |
R-factor | 0.15526 |
Rwork | 0.154 |
R-free | 0.18359 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hqk |
RMSD bond length | 0.006 |
RMSD bond angle | 1.541 |
Data reduction software | DENZO |
Data scaling software | PROTEUM PLUS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.048 | 0.443 |
Number of reflections | 26145 | |
<I/σ(I)> | 14.7 | 3.3 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 100 mM HEPES, 22% PEG 3000 |