Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.970, 59.550, 46.350 |
| Unit cell angles | 90.00, 103.81, 90.00 |
Refinement procedure
| Resolution | 34.221 - 1.961 |
| R-factor | 0.1863 |
| Rwork | 0.185 |
| R-free | 0.21890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pvg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.826 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 69.419 | 45.010 | 2.070 |
| High resolution limit [Å] | 1.961 | 6.200 | 1.961 |
| Rmerge | 0.033 | 0.384 | |
| Rmeas | 0.083 | 0.040 | 0.456 |
| Rpim | 0.044 | 0.022 | 0.243 |
| Number of reflections | 27064 | 835 | 3928 |
| <I/σ(I)> | 8.7 | 15.3 | 1.9 |
| Completeness [%] | 99.2 | 92.1 | 99.5 |
| Redundancy | 3.5 | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 0.1 M Tris-HCl, 10-20% PEG 4000, 0.2 M Na-acetate |
